Journal: Scientific Reports

Article Title: Differential epigenetic regulation between the alternative promoters, PRDM1α and PRDM1β , of the tumour suppressor gene PRDM1 in human multiple myeloma cells

doi: 10.1038/s41598-020-72946-z

Figure Lengend Snippet: Schematic representation of the regulation of human PRDM1 promoters in multiple myeloma. a Factors such as SP1, SP3 and EGR1 act on the PRDM1α promoter, inducing its transcription , the opposite effect as that of PAX5 . On the PRDM1β promoter, TP53 (as an activator) , and FOX factors (as inhibitors) can regulate PRDM1β transcription. Regarding epigenetic regulation, methylation seems to play a decisive role in PRDM1β expression but not in PRDM1α expression. In the case of histone modifications, H3K4me1 and H3K9me2 marks seem to be linked to the repression of the PRDM1β promoter. Activators and inhibitors are represented above and under the DNA helix, respectively. Filled and open lollipops represent the methylation and demethylation status of the promoter, respectively. This figure was created using Corel Draw 2018 version 20.1.0.708 software (Corel Corporation, Ottawa, Canada). b Schematic representation showing how PRDM1β expression tilts the balance in favour of proliferation due to a diminished apoptosis rate. Inhibiting PRDM1β expression using drugs targeting epigenetic elements at its promoter may restore the balance to maintain normal PC cell fate.

Article Snippet: This figure was created using Corel Draw 2018 version 20.1.0.708 software (Corel Corporation, Ottawa, Canada). b Schematic representation showing how PRDM1β expression tilts the balance in favour of proliferation due to a diminished apoptosis rate.

Techniques: Methylation, Expressing, Software

Journal: Scientific Reports

Article Title: Coexisting with sharks: a novel, socially acceptable and non-lethal shark mitigation approach

doi: 10.1038/s41598-020-74270-y

Figure Lengend Snippet: Shark analogue detection probabilities in ( a ) sunny (n = 29 analogue deployments over 5 days) and ( b ) cloudy (n = 22 analogue deployments over 5 days) conditions at Surf Beach in Kiama, NSW, Australia. Maps were created using ArcGIS Pro version 2.0.1 by Esri ( www.esri.com ).

Article Snippet: Maps were created using ArcGIS Pro version 2.0.1 by Esri ( www.esri.com ).

Techniques:

Journal: Scientific Reports

Article Title: Coexisting with sharks: a novel, socially acceptable and non-lethal shark mitigation approach

doi: 10.1038/s41598-020-74270-y

Figure Lengend Snippet: Surf Beach, Kiama is located on the south coast of NSW Australia. The beach is typical of a sandy coastal embayment and is enclosed by two fringing rocky reef headlands. Swimmers and surfers are frequent users of the bay over summer, with usage regulated by lifeguards. Swimmers are encouraged to swim within a flagged area where surfers are prohibited. Maps were created using ArcGIS Pro version 2.0.1 by Esri ( www.esri.com ).

Article Snippet: Maps were created using ArcGIS Pro version 2.0.1 by Esri ( www.esri.com ).

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Development of Optical Sensors Based on Neutral Red Absorbance for Real-Time pH Measurements

doi: 10.3390/s24175610

Figure Lengend Snippet: Optimized mirror geometry in 316 L stainless steel, using Zemax/optic studio software (version 20.1).

Article Snippet: The mirror’s geometry was optimized using Zemax/optic studio software (version 20.1).

Techniques: Software

Journal: Journal of Virology

Article Title: H5N1 Virus-Like Particle Vaccine Elicits Cross-Reactive Neutralizing Antibodies That Preferentially Bind to the Oligomeric Form of Influenza Virus Hemagglutinin in Humans

doi: 10.1128/jvi.05406-11

Figure Lengend Snippet: FIG. 2. Characterization of bacterially expressed and purified H5N1 HA proteins. (A and B) Characterization of purified H5N1 HA proteins from E. coli by Superdex S-200 gel filtration chromatography. Purified H5N1 HA1 proteins with an intact N terminus (positions 1 to 320) (A) and HA1 with an N-terminal deletion (28 to 320) (B) were subjected to gel filtration. The panels show superimposed elution profiles of purified HA1 proteins (red lines) overlaid with calibration standards (gray lines). The elution volumes of protein species are shown in parentheses. (C and D) Steady-state binding equilibrium analysis of conformation-dependent human H5N1 neutralizing MAbs FLD21.40 (C) and FLA3.14 (D) at 10 g/ml to purified bacterially expressed H5N1 HA1 proteins immobilized on a sensor chip through the free amine group and on a blank flow cell, free of peptide. Binding was recorded using a ProteOn system surface plasmon resonance biosensor instrument (Bio-Rad Labs, Hercules, CA). (E) Agglutination of human RBCs by properly folded oligomeric H5N1 rHA1(1-320) protein and its monomeric H5N1 rHA1(28-320) counterpart along with rgH5N1 virus. Serial dilutions of purified HA1 proteins were mixed with washed RBCs, and hemagglutination was read after 30 min at room temperature. Reassorted virus rgH5N1xPR8 (2:6) A/Vietnam/1203/2004 (clade 1) was used as a positive control.

Article Snippet: Binding kinetics for the selected human vaccine sera and data analyses were calculated using Bio-Rad ProteOn manager software (version 2.0.1).

Techniques: Chromatography, Binding Assay, SPR Assay, Agglutination, Virus, Positive Control